Culex quinquefasciatus

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Culex quinquefasciatus

The Johannesburg (JHB) strain is the source of genomic DNA for the Culex quinquefasciatus genome project. This strain has been selected because it consistently yields high quality chromosome spreads suitable for in situ hybridization studies [1]. This strain is also insecticide susceptible and so can be transported and maintained in laboratories world-wide. The Johannesburg (JHB) strain was established from offspring reared from 20 egg rafts collected in a single unused fish pond in March 2001. Population genetic studies revealed that the Johannesburg C. quinquefasciatus population was genetically isolated from a sympatric C. pipiens pipiens population [2]. Consequently, the JHB strain is expected to possess a high degree of genetic divergence from C. pipiens pipiens. Based on many generations of inbreeding at relatively small population size (often no more than about 50 females per generation), the colony is expected to show relatively little genetic variation [3]. In addition to having excellent polytene chromosomes in larval salivary gland tissues, this colony has also been found to be free of any visible polytene chromosome inversions.

Current release

  Release
Assembly CpipJ1
Geneset CpipJ1.2

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